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Human stem cell manual : a laborator...
~
Loring, Jeanne Frances (1950-)
Human stem cell manual : a laboratory guide
紀錄類型:
書目-語言資料,印刷品 : 單行本
副題名:
a laboratory guide
其他作者:
LoringJeanne Frances, 1950-
其他作者:
SchwartzPhilip H.,
其他作者:
WesselschmidtRobin L.,
其他團體作者:
ScienceDirect (Online service)
出版地:
Amsterdam
出版者:
Academic Press;
出版年:
2007.
面頁冊數:
xxiii, 461 p.ill. (some col.) : 26 cm.;
標題:
Biology - Research -
標題:
Embryonic stem cells. -
標題:
Stem cells -
標題:
Electronic books. -
標題:
Adult Stem Cells. -
標題:
Biomedical Research - methods. -
標題:
Embryonic Stem Cells. -
標題:
Stem Cells -
電子資源:
http://www.sciencedirect.com/science/book/9780123704658
附註:
Electronic reproduction. Amsterdam : Elsevier Science & Technology, 2007.
摘要註:
Stem cells are self-replicating and undifferentiated, meaning their function is not yet cell, tissue, or organ-specific. Due to the unique nature of these cells, research into their biology and function holds great promise for therapeutic applications through replacement or repair of diseased and damaged cells. This reader-friendly manual provides a practical "hands on" guide to the culture of human embryonic and somatic stem cells. By presenting methods for embryonic and adult lines side-by-side, the authors lay out an elegant and unique path to understanding the science of stem cell practice. The authors begin with a broad-based introduction to the field, and also review legal and regulatory issues and patents. Each experimental strategy is presented with an historical introduction, detailed method, discussion of alternative methods, and common pitfalls. This lab guide for researchers also serves as a textbook for undergraduate and graduate students in laboratory courses.
ISBN:
0123704650
內容註:
Basic methods in stem cell culture Characterization of stem cells Differentiation of human embryonic cells Genetic manipulation of stem cells Advanced methods Setting up a laboratory Stem cells and society.
內容註:
Foreword by James Battey Foreword by Jeannie Fontana Preface Part I: Basic methods: Maintenance of stem cell cultures 1.Human Embryonic Stem Cell Culture 2.Feeder layers and feeder-free culture 3.Mouse Embryonic Fibroblast Feeder Cells 4.Cryopreservation of hESC Part II: Basic methods: Characterization of stem cells 5.Classical Cytogenetics: Karyotyping 6.Spectral Karyotyping and Fluorescent in situ Hybridization 7.Using SNP Genotyping to Identify Cell Lines, Determine Genomic Abnormalities and Monitor DNA Methylation Patterns 8.FACS/Cell sorting 9.Fluorescence Immunocytochemical Analysis of Stem Cells 10.Characterization of Stem Cells Using RT-PCR 11.Gene Expression Profiling of Stem Cells by Microarray 12.Teratomas produced from Human Embryonic Stem Cells Xenografted into Immunodeficient Mice 13.Generation of HESC-Derived Teratomas Part III: Differentiation of stem cells 14.Embryoid Body Formation and Neuroepithelia Differentiation 15.Motor Neuron and Dopamine Neuron Differentiation 16.Oligodendrocyte Differentiation from hESC 17.Cardiac 18.Hematopoietic Part IV: Genetic manipulation of stem cells 19.Genetic Manipulation of hESC: Lentivirus Vectors 20.Methods developed for mouse ES cells Part V: Advanced methods 21.Derivation of human ES cells 22.Neural Stem Cell Culture 23.Transplantation of stem cells 24.In vitro fertilization 25.Functional characterization-neural cells Part VI: Practical issues in establishing a human ES cell laboratory 26.Setting Up a Facility for hESC Research Part VII: Essays on stem cell biology 27.Intellectual Property: Owning the Stem Cell 28.Ethical Concerns for Stem Cell Research 29.Guidelines for hESC Research Oversight (ESCRO) Committees.
Human stem cell manual : a laboratory guide
Human stem cell manual
: a laboratory guide / edited by Jeanne F. Loring, Robin L. Wesselschmidt, Philip H. Schwartz. - Amsterdam : Academic Press, 2007.. - xxiii, 461 p. ; ill. (some col.) ; 26 cm..
Basic methods in stem cell cultureForeword by James Battey.
Electronic reproduction. Amsterdam : Elsevier Science & Technology, 2007..
Includes bibliographical references and index..
ISBN 0123704650ISBN 9780123704658
Biology Embryonic stem cells.Stem cellsElectronic books.Adult Stem Cells.Biomedical Research Embryonic Stem Cells.Stem Cells -- Research -- methods.
Loring, Jeanne Frances
Human stem cell manual : a laboratory guide
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An electronic book accessible through the World Wide Web; click for information
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